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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AF2190 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Carbonic Anhydrase XII/CA12 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse Carbonic Anhydrase XII/CA12 is observed and less than 5% cross-reactivity with recombinant human CA1, 3, 5A, 5B, 6, 7, 8, and 10 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O43570- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Carbonic anhydrase IX promotes tumor growth and necrosis in vivo and inhibition enhances anti-VEGF therapy.
McIntyre A, Patiar S, Wigfield S, Li JL, Ledaki I, Turley H, Leek R, Snell C, Gatter K, Sly WS, Vaughan-Jones RD, Swietach P, Harris AL
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jun 1;18(11):3100-11
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jun 1;18(11):3100-11
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Carbonic Anhydrase XII/CA12 by Western Blot. Western blot shows lysates of human kidney tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Carbonic Anhydrase XII/CA12 by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately 60 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
