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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- TA504366 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504366, RRID:AB_11126100
- Product name
- DPP8 mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)
- Antibody type
- Monoclonal
- Description
- DPP8 mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- DPP8
- Isotype
- IgG
- Antibody clone number
- OTI1D2
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Establishment of a dipeptidyl peptidases (DPP) 8/9 expressing cell model for evaluating the selectivity of DPP4 inhibitors.
Huan Y, Jiang Q, Liu JL, Shen ZF
Journal of pharmacological and toxicological methods 2015 Jan-Feb;71:8-12
Journal of pharmacological and toxicological methods 2015 Jan-Feb;71:8-12
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP8 (RC214685, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP8.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DPP8 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-DPP8 monoclonal antibody. (1:200)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western blot analysis of protein levels of DPP4/8/9 in 293E cells transfected with pcDNA3.1 (Mock), pcDNA3.1-hDPP8 (DPP8) and pcDNA3.1-hDPP9 (DPP9), the purified recombinant human DPP4 protein (rhDPP4) was used as a positive control for the DPP4 antibody, antibodies targeting human DPP4/8/9 (TA500733/TA504366/TA504040) and ?-actin (TA310155) were purchased from OriGene.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPP8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504366)
- Validation comment
- IHC
