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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA501055 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501055, RRID:AB_11129402
- Product name
- Anti-RIC8A (RIC8) mouse monoclonal antibody, clone OTI3G3 (formerly 3G3)
- Antibody type
- Monoclonal
- Description
- Anti-RIC8A (RIC8) mouse monoclonal antibody, clone OTI3G3 (formerly 3G3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- RIC8A
- Isotype
- IgG
- Antibody clone number
- OTI3G3
- Vial size
- 100 µl
- Concentration
- 0.83 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8A (RC211359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIC8A.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RIC8A monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-RIC8A mouse monoclonal antibody (TA501055) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8A(RC211359).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-RIC8A mouse monoclonal antibody (TA501055).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-RIC8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501055, Dilution 1:50)
- Validation comment
- IHC
