15261-1-AP

antibody from Invitrogen Antibodies

Targeting: PSMD13 p40.5, Rpn9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15261-1-AP

Provider

Invitrogen Antibodies

Product name

PSMD13 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MKDVPGFLQ QSQSSGPGQP AVWHRLEELY TKKLWHQLTL QVLDFVQDPC FAQGDGLIKL YENFISEFEH RVNPLSLVEI ILHVVRQMTD PNVALTFLEK TREKVKSSDE AVILCKTAIG ALKLNIGDLQ VTKETIEDVE EMLNNLPGVT SVHSRFYDLS SKYYQTIGNH ASYYKDALRF LGCVDIKDLP VSEQQERAFT LGLAGLLGEG VFNFGELLMH PVLESLRNTD RQWLIDTLYA FNSGNVERFQ TLKTAWGQQP DLAANEAQLL RKIQLLCLME MTFTRPANHR QLTFEEIAKS AKITVNEVEL LVMKALSVGL VKGSIDEVDK RVHMTWVQPR VLDLQQIKGM KDRLEFWCTD VKSMEMLVEH QAHDILT (1-376 aa encoded by BC001100)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 15261-1-AP (PSMD13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 15261-1-AP (PSMD13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 15261-1-AP (PSMD13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using PSMD13 antibody 15261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 15261-1-AP (PSMD13 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 15261-1-AP (PSMD13 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 15261-1-AP ( PSMD13 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 15261-1-AP ( PSMD13 Antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PSMD13 (IP:15261-1-AP, 4ug; Detection:15261-1-AP 1:1000) with HEK-293T cells lysate 4000ug.