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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- M02356 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-PI 3 Kinase Class 3 PIK3C3 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for VPS34/PIK3C3 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human, Mouse, Rat VPS34/PIK3C3 information: Molecular Weight: 101549 MW; Subcellular Localization: Midbody . Late endosome . Cytoplasmic vesicle, autophagosome . As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (Probable). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity); Tissue Specificity: Ubiquitously expressed, with a highest expression in skeletal muscle.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- CDO-16
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney, using PI 3 Kinase Class 3 Antibody(M02356)PIK3C3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PIK3C3 Antibody (M02356)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
