Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN1449618 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Phosphoinositide-3-Kinase, Class 3 (PIK3C3) (AA 19-49) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 19~49 amino acids surrounding Serine 34 of Human PI3KC3
- Description
- Protein A column, followed by peptide affinity purification.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 19-49
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Membrane phospholipid metabolism during phagocytosis in human neutrophils.
Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment.
Tuberculosis toxin blocking phagosome maturation inhibits a novel Ca2+/calmodulin-PI3K hVPS34 cascade.
A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system.
Minakami R, Maehara Y, Kamakura S, Kumano O, Miyano K, Sumimoto H
Genes to cells : devoted to molecular & cellular mechanisms 2010 May;15(5):409-24
Genes to cells : devoted to molecular & cellular mechanisms 2010 May;15(5):409-24
Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment.
Gao W, Kang JH, Liao Y, Ding WX, Gambotto AA, Watkins SC, Liu YJ, Stolz DB, Yin XM
The Journal of biological chemistry 2010 Jan 8;285(2):1371-83
The Journal of biological chemistry 2010 Jan 8;285(2):1371-83
Tuberculosis toxin blocking phagosome maturation inhibits a novel Ca2+/calmodulin-PI3K hVPS34 cascade.
Vergne I, Chua J, Deretic V
The Journal of experimental medicine 2003 Aug 18;198(4):653-9
The Journal of experimental medicine 2003 Aug 18;198(4):653-9
A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system.
Volinia S, Dhand R, Vanhaesebroeck B, MacDougall LK, Stein R, Zvelebil MJ, Domin J, Panaretou C, Waterfield MD
The EMBO journal 1995 Jul 17;14(14):3339-48
The EMBO journal 1995 Jul 17;14(14):3339-48
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of PI3KC3 Antibody Cat.-No AP32186PU-N in Hela cell line lysates (35ug/lane). PI3KC3 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PI3KC3 Antibody Cat.-No AP32186PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.