H00000108-M01

antibody from Abnova Corporation

Targeting: ADCY2 AC2, HBAC2, KIAA1060

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000108-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000108-M01, RRID:AB_605906

Product name

ADCY2 monoclonal antibody (M01), clone 1D4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ADCY2.

Antigen sequence

GKLDAINKHSFNDFKLRVGINHGPVIAGVIGAQKP
QYDIWGNTVNVASRMDSTGVLDKIQVTEETSLVLQ
TLGYTCTCRGIINVKGKGDLKTYFVNTEMSRSLSQ
SNVAS


Isotype

IgG

Antibody clone number

1D4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ADCY2 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between GNAS and ADCY2. HeLa cells were stained with anti-GNAS rabbit purified polyclonal 1:1200 and anti-ADCY2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)