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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-116409 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UT-A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.45 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of UT-A1 using the UT-A1 Polyclonal Antibody (Product # PA5-116409) at a 1:500 dilution. Lane 1: 293T/17 whole cell lysate, Lane 2: Human brain lysate, Lane 3: K562 whole cell lysate 293T/17 whole cell lysate at 30 µg per lane. H. brain lysate at 50 µg per lane. K562 whole cell lysate at 40 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 101 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of UT-A1 using the UT-A1 Polyclonal Antibody (Product # PA5-116409) at a 1:500 dilution. Human testis lysates were loaded at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 101 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of UT-A1 in paraffin-embedded human brain tissue using the UT-A1 Polyclonal Antibody (Product # PA5-116409) at a dilution of 1:500. Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
