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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 13382-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCAPD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP ( NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using NCAPD2 antibody 13382-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13382-1-AP (NCAPD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NCAPD2 (IP:13382-1-AP, 4ug; Detection:13382-1-AP 1:600) with HEK-293 cells lysate 2800ug.
