PA5-28893

antibody from Invitrogen Antibodies

Targeting: MCAM CD146, HEMCAM, MelCAM, METCAM, MUC18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28893

Provider

Invitrogen Antibodies

Product name

CD146 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HUVEC, rat liver extract. Predicted reactivity: Mouse (81%), Rat (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.21 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Mel-CAM/CD146 using 30 µg of HCT116 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a Mel-CAM/CD146 polyclonal antibody (Product # PA5-28893) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CD146 was performed by separating 30 µg of HUVEC whole cell lysates by 7.5 % SDS-PAGE. Proteins were transferred to a membrane and probed with a CD146 Polyclonal Antibody (Product # PA5-28893) at a dilution of 1:10000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD146 Polyclonal Antibody (Product # PA5-28893). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 Polyclonal Antibody (Product # PA5-28893) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD146 Polyclonal Antibody detects CD146 protein by western blot analysis. A. 50 µg rat liver extract.7.5 % SDS-PAGE. CD146 Polyclonal Antibody (Product # PA5-28893) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CD146 was performed in HUVEC cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CD146 Polyclonal Antibody (Product # PA5-28893) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD146 Polyclonal Antibody detects CD146 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse placenta. Green: CD146 stained by CD146 Polyclonal Antibody (Product # PA5-28893) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD146 Polyclonal Antibody detects CD146 protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CD146 Polyclonal Antibody (Product # PA5-28893) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD146 Polyclonal Antibody detects CD146 protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CD146 Polyclonal Antibody (Product # PA5-28893) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146 (Product # PA5-28893) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.