NB100-1985

antibody from Novus Biologicals

Targeting: CD74 DHLAG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-1985

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1985, RRID:AB_10001511

Product name

Mouse Monoclonal CD74 Antibody

Antibody type

Monoclonal

Description

Protein G purified. This detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.

Reactivity

Human, Mouse

Host

Mouse

Antigen sequence

The epitope is in a region of the c
ytoplasmic tail of human CD74 which
is common to all isoforms.


Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Cell Surface CD74-MIF Interactions Drive Melanoma Survival in Response to Interferon-γ.

Tanese K, Hashimoto Y, Berkova Z, Wang Y, Samaniego F, Lee JE, Ekmekcioglu S, Grimm EA
The Journal of investigative dermatology 2015 Nov;135(11):2775-2784

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Gain in brain immunity in the oldest-old differentiates cognitively normal from demented individuals.

Katsel P, Tan W, Haroutunian V
PloS one 2009 Oct 29;4(10):e7642

---

Assembly and transport properties of invariant chain trimers and HLA-DR-invariant chain complexes.

Lamb CA, Cresswell P
Journal of immunology (Baltimore, Md. : 1950) 1992 Jun 1;148(11):3478-82

---

Formation of a nine-subunit complex by HLA class II glycoproteins and the invariant chain.

Roche PA, Marks MS, Cresswell P
Nature 1991 Dec 5;354(6352):392-4

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Ramos whole cell protein was separated on a 4-12% gel by SDS-PAGE and protein transferred to PVDF. The membrane was probed with anti-CD74 at 2 ug/ml and detected with and anti-mouse HRP secondary antibody using chemiluminescence. The arrow head shows the detection of CD74.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human spleen using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human renal cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD74 Antibody (PIN.1) [NB100-1985] - CD74 in human PBMCs (gated on all monocytes). Orange represents isotype control and blue represents anti-CD74 antibody staining.