AF1258

antibody from Novus Biologicals

Targeting: IL25 IL-17E, IL-25, IL17E

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1258

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-17E/IL-25 Antibody

Antibody type

Polyclonal

References

Submitted references

IL-25 promotes Th2 bias by upregulating IL-4 and IL-10 expression of decidual γδT cells in early pregnancy.

Zhang Y, Wang Y, Li MQ, Duan J, Fan DX, Jin LP
Experimental and therapeutic medicine 2018 Feb;15(2):1855-1862

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell CXCL1/GRO Alpha Secretion Induced by IL-17E/IL-25 and Neutralization by Human IL-17E/IL-25 Antibody. Recombinant Human IL-17E/ IL-25 induces CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha Duoset (Catalog # DY275). Under these conditions, CXCL1/GRO alpha secretion elicited by IL-17E/IL-25 is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17E/IL-25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1258). The ND₅₀ is typically 0.04-0.24 ug/mL.