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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF1258 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17E/IL-25 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-17E in direct ELISAs and Western blots. In direct ELISAs, less than 60% cross-reactivity with recombinant mouse IL-17E is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9H293- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-25 promotes Th2 bias by upregulating IL-4 and IL-10 expression of decidual γδT cells in early pregnancy.
Zhang Y, Wang Y, Li MQ, Duan J, Fan DX, Jin LP
Experimental and therapeutic medicine 2018 Feb;15(2):1855-1862
Experimental and therapeutic medicine 2018 Feb;15(2):1855-1862
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell CXCL1/GRO Alpha Secretion Induced by IL-17E/IL-25 and Neutralization by Human IL-17E/IL-25 Antibody. Recombinant Human IL-17E/ IL-25 induces CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha Duoset (Catalog # DY275). Under these conditions, CXCL1/GRO alpha secretion elicited by IL-17E/IL-25 is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17E/IL-25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1258). The ND50 is typically 0.04-0.24 ug/mL.
