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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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Validation data
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- Product number
- H00006050-M07 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006050-M07, RRID:AB_1137011
- Product name
- RNH1 monoclonal antibody (M07), clone 3F5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant RNH1.
- Antigen sequence
MSLDIQSLDIQCEELSDARWAELLPLLQQCQVVRL
DDCGLTEARCKDISSALRVNPALAELNLRSNELGD
VGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVL
SSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDP
QCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKEL
TVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCG
VTSDNCRDLCGIVASKASLRELALGSNKLGDVGMA
ELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVL
RAKESLKELSLAGNELGDEGARLLCETLLEPGCQL
ESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISN
NRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDS
SCSSLAATLLANHSLRELDLSNNCLGDAGILQLVE
SVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKP
SLRVIS- Isotype
- IgG
- Antibody clone number
- 3F5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The ribonuclease/angiogenin inhibitor is also present in mitochondria and nuclei.
Furia A, Moscato M, Calì G, Pizzo E, Confalone E, Amoroso MR, Esposito F, Nitsch L, D'Alessio G
FEBS letters 2011 Feb 18;585(4):613-7
FEBS letters 2011 Feb 18;585(4):613-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RNH1 expression in transfected 293T cell line by RNH1 monoclonal antibody (M07), clone 3F5.Lane 1: RNH1 transfected lysate(50 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RNH1 monoclonal antibody (M07), clone 3F5. Western Blot analysis of RNH1 expression in K-562.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RNH1 monoclonal antibody (M07), clone 3F5. Western Blot analysis of RNH1 expression in human colon.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RNH1 is 10 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
