Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [10]
- Flow cytometry [2]
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Validation data
Reference
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- Product number
- TA501875 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501875, RRID:AB_11124610
- Product name
- RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11)
- Antibody type
- Monoclonal
- Description
- RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- RNH1
- Isotype
- IgG
- Antibody clone number
- OTI3F11
- Vial size
- 100 µl
- Concentration
- 0.94 mg/ml
Submitted references Endogenous RNase inhibitor contributes to stability of RNA in crude cell lysates: Applicability to RT-qPCR.
Wang X, Teferedegne B, Shatzkes K, Tu W, Murata H
Analytical biochemistry 2016 Nov 15;513:21-27
Analytical biochemistry 2016 Nov 15;513:21-27
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNH1 (RC208360, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNH1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RNH1 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RNH1 mouse monoclonal antibody. (TA501875)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RNH1 mouse monoclonal antibody. (TA501875)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RNH1 mouse monoclonal antibody. (TA501875)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RNH1 mouse monoclonal antibody. (TA501875)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RNH1 mouse monoclonal antibody. (TA501875)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-RNH1 antibody(TA501875),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC208360 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNH1 antibody(TA501875), and then analyzed by flow cytometry.
- Validation comment
- FC