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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [3]
- ELISA [1]
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- Product number
- H00006050-M07 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006050-M07, RRID:AB_1200055
- Product name
- Mouse Monoclonal Ribonuclease Inhibitor Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RNH1 - ribonuclease/angiogenin inhibitor 1 (3F5)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The ribonuclease/angiogenin inhibitor is also present in mitochondria and nuclei.
Furia A, Moscato M, Calì G, Pizzo E, Confalone E, Amoroso MR, Esposito F, Nitsch L, D'Alessio G
FEBS letters 2011 Feb 18;585(4):613-7
FEBS letters 2011 Feb 18;585(4):613-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Analysis of RNH1 expression in transfected 293T cell line by RNH1 monoclonal antibody (M07), clone 3F5. Lane 1: RNH1 transfected lysatE (50 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Analysis of RNH1 expression in human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Analysis of RNH1 expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Ribonuclease Inhibitor Antibody (3F5) [H00006050-M07] - Detection limit for recombinant GST tagged RNH1 is 10 ng/ml as a capture antibody.
