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Learn11517-1-AP
antibody from Proteintech Group
Targeting: CENPJ
BM032, CPAP, LAP, LIP1, MCPH6, Sas-4, SASS4, SCKL4
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [39]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 11517-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11517-1-AP, RRID:AB_2244605
- Product name
- CENPJ antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CENPJ antibody (Cat. #11517-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.
Modifier Genes in Microcephaly: A Report on WDR62, CEP63, RAD50 and PCNT Variants Exacerbating Disease Caused by Biallelic Mutations of ASPM and CENPJ.
Loss of the centrosomal protein Cenpj leads to dysfunction of the hypothalamus and obesity in mice.
Centrosomal P4.1-associated protein (CPAP) positively regulates endocytic vesicular transport and lysosome targeting of EGFR.
Centriole and PCM cooperatively recruit CEP192 to spindle poles to promote bipolar spindle assembly.
Loss of CPAP causes sustained EGFR signaling and epithelial-mesenchymal transition in oral cancer.
TRIM37 controls cancer-specific vulnerability to PLK4 inhibition.
The association of microcephaly protein WDR62 with CPAP/IFT88 is required for cilia formation and neocortical development.
Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.
CEP135 isoform dysregulation promotes centrosome amplification in breast cancer cells.
Cenpj Regulates Cilia Disassembly and Neurogenesis in the Developing Mouse Cortex.
Albatross/FBF1 contributes to both centriole duplication and centrosome separation.
PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN.
Centrobin controls primary ciliogenesis in vertebrates.
NEK7 is required for G1 progression and procentriole formation.
Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.
Autophagy controls centrosome number by degrading Cep63.
Role for the IFT-A Complex in Selective Transport to the Primary Cilium.
Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation.
53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration.
Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole.
Promotion and Suppression of Centriole Duplication Are Catalytically Coupled through PLK4 to Ensure Centriole Homeostasis.
A Short CEP135 Splice Isoform Controls Centriole Duplication.
De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly.
MDM1 is a microtubule-binding protein that negatively regulates centriole duplication.
Centrobin-mediated regulation of the centrosomal protein 4.1-associated protein (CPAP) level limits centriole length during elongation stage.
Cenpj/CPAP regulates progenitor divisions and neuronal migration in the cerebral cortex downstream of Ascl1.
Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4.
RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity.
Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation.
Centrobin-centrosomal protein 4.1-associated protein (CPAP) interaction promotes CPAP localization to the centrioles during centriole duplication.
PIPKIγ targets to the centrosome and restrains centriole duplication.
SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication.
Stabilization of cartwheel-less centrioles for duplication requires CEP295-mediated centriole-to-centrosome conversion.
A novel tankyrase small-molecule inhibitor suppresses APC mutation-driven colorectal tumor growth.
CEP120 and SPICE1 cooperate with CPAP in centriole elongation.
The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.
Interaction proteomics identify NEURL4 and the HECT E3 ligase HERC2 as novel modulators of centrosome architecture.
Tankyrase 1 regulates centrosome function by controlling CPAP stability.
Stemm-Wolf AJ, O'Toole ET, Sheridan RM, Morgan JT, Pearson CG
Molecular biology of the cell 2021 Oct 1;32(20):ar4
Molecular biology of the cell 2021 Oct 1;32(20):ar4
Modifier Genes in Microcephaly: A Report on WDR62, CEP63, RAD50 and PCNT Variants Exacerbating Disease Caused by Biallelic Mutations of ASPM and CENPJ.
Makhdoom EUH, Waseem SS, Iqbal M, Abdullah U, Hussain G, Asif M, Budde B, Höhne W, Tinschert S, Saadi SM, Yousaf H, Ali Z, Fatima A, Kaygusuz E, Khan A, Jameel M, Khan S, Tariq M, Anjum I, Altmüller J, Thiele H, Höning S, Baig SM, Nürnberg P, Hussain MS
Genes 2021 May 13;12(5)
Genes 2021 May 13;12(5)
Loss of the centrosomal protein Cenpj leads to dysfunction of the hypothalamus and obesity in mice.
Ding W, Zhang C, Wang B, Zhou X, Sun L, Zhong S, Liu J, Zhang J, Wang X, Wu Q
Science China. Life sciences 2021 Mar;64(3):419-433
Science China. Life sciences 2021 Mar;64(3):419-433
Centrosomal P4.1-associated protein (CPAP) positively regulates endocytic vesicular transport and lysosome targeting of EGFR.
Gudi R, Palanisamy V, Vasu C
Scientific reports 2021 Jun 16;11(1):12689
Scientific reports 2021 Jun 16;11(1):12689
Centriole and PCM cooperatively recruit CEP192 to spindle poles to promote bipolar spindle assembly.
Chinen T, Yamazaki K, Hashimoto K, Fujii K, Watanabe K, Takeda Y, Yamamoto S, Nozaki Y, Tsuchiya Y, Takao D, Kitagawa D
The Journal of cell biology 2021 Feb 1;220(2)
The Journal of cell biology 2021 Feb 1;220(2)
Loss of CPAP causes sustained EGFR signaling and epithelial-mesenchymal transition in oral cancer.
Gudi RR, Janakiraman H, Howe PH, Palanisamy V, Vasu C
Oncotarget 2021 Apr 13;12(8):807-822
Oncotarget 2021 Apr 13;12(8):807-822
TRIM37 controls cancer-specific vulnerability to PLK4 inhibition.
Meitinger F, Ohta M, Lee KY, Watanabe S, Davis RL, Anzola JV, Kabeche R, Jenkins DA, Shiau AK, Desai A, Oegema K
Nature 2020 Sep;585(7825):440-446
Nature 2020 Sep;585(7825):440-446
The association of microcephaly protein WDR62 with CPAP/IFT88 is required for cilia formation and neocortical development.
Shohayeb B, Ho U, Yeap YY, Parton RG, Millard SS, Xu Z, Piper M, Ng DCH
Human molecular genetics 2020 Jan 15;29(2):248-263
Human molecular genetics 2020 Jan 15;29(2):248-263
Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.
Holdgaard SG, Cianfanelli V, Pupo E, Lambrughi M, Lubas M, Nielsen JC, Eibes S, Maiani E, Harder LM, Wesch N, Foged MM, Maeda K, Nazio F, de la Ballina LR, Dötsch V, Brech A, Frankel LB, Jäättelä M, Locatelli F, Barisic M, Andersen JS, Bekker-Jensen S, Lund AH, Rogov VV, Papaleo E, Lanzetti L, De Zio D, Cecconi F
Nature communications 2019 Sep 13;10(1):4176
Nature communications 2019 Sep 13;10(1):4176
CEP135 isoform dysregulation promotes centrosome amplification in breast cancer cells.
Ganapathi Sankaran D, Stemm-Wolf AJ, Pearson CG
Molecular biology of the cell 2019 May 1;30(10):1230-1244
Molecular biology of the cell 2019 May 1;30(10):1230-1244
Cenpj Regulates Cilia Disassembly and Neurogenesis in the Developing Mouse Cortex.
Ding W, Wu Q, Sun L, Pan NC, Wang X
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Mar 13;39(11):1994-2010
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Mar 13;39(11):1994-2010
Albatross/FBF1 contributes to both centriole duplication and centrosome separation.
Inoko A, Yano T, Miyamoto T, Matsuura S, Kiyono T, Goshima N, Inagaki M, Hayashi Y
Genes to cells : devoted to molecular & cellular mechanisms 2018 Dec;23(12):1023-1042
Genes to cells : devoted to molecular & cellular mechanisms 2018 Dec;23(12):1023-1042
PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN.
Sydor AM, Coyaud E, Rovelli C, Laurent E, Liu H, Raught B, Mennella V
eLife 2018 Aug 31;7
eLife 2018 Aug 31;7
Centrobin controls primary ciliogenesis in vertebrates.
Ogungbenro YA, Tena TC, Gaboriau D, Lalor P, Dockery P, Philipp M, Morrison CG
The Journal of cell biology 2018 Apr 2;217(4):1205-1215
The Journal of cell biology 2018 Apr 2;217(4):1205-1215
NEK7 is required for G1 progression and procentriole formation.
Gupta A, Tsuchiya Y, Ohta M, Shiratsuchi G, Kitagawa D
Molecular biology of the cell 2017 Jul 15;28(15):2123-2134
Molecular biology of the cell 2017 Jul 15;28(15):2123-2134
Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.
Jayaraman D, Kodani A, Gonzalez DM, Mancias JD, Mochida GH, Vagnoni C, Johnson J, Krogan N, Harper JW, Reiter JF, Yu TW, Bae BI, Walsh CA
Neuron 2016 Nov 23;92(4):813-828
Neuron 2016 Nov 23;92(4):813-828
Autophagy controls centrosome number by degrading Cep63.
Watanabe Y, Honda S, Konishi A, Arakawa S, Murohashi M, Yamaguchi H, Torii S, Tanabe M, Tanaka S, Warabi E, Shimizu S
Nature communications 2016 Nov 21;7:13508
Nature communications 2016 Nov 21;7:13508
Role for the IFT-A Complex in Selective Transport to the Primary Cilium.
Fu W, Wang L, Kim S, Li J, Dynlacht BD
Cell reports 2016 Nov 1;17(6):1505-1517
Cell reports 2016 Nov 1;17(6):1505-1517
Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation.
Kim JH, Ki SM, Joung JG, Scott E, Heynen-Genel S, Aza-Blanc P, Kwon CH, Kim J, Gleeson JG, Lee JE
Biochimica et biophysica acta 2016 Jun;1863(6 Pt A):1307-18
Biochimica et biophysica acta 2016 Jun;1863(6 Pt A):1307-18
53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration.
Meitinger F, Anzola JV, Kaulich M, Richardson A, Stender JD, Benner C, Glass CK, Dowdy SF, Desai A, Shiau AK, Oegema K
The Journal of cell biology 2016 Jul 18;214(2):155-66
The Journal of cell biology 2016 Jul 18;214(2):155-66
Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole.
Tsuchiya Y, Yoshiba S, Gupta A, Watanabe K, Kitagawa D
Nature communications 2016 Aug 26;7:12567
Nature communications 2016 Aug 26;7:12567
Promotion and Suppression of Centriole Duplication Are Catalytically Coupled through PLK4 to Ensure Centriole Homeostasis.
Kim M, O'Rourke BP, Soni RK, Jallepalli PV, Hendrickson RC, Tsou MB
Cell reports 2016 Aug 2;16(5):1195-1203
Cell reports 2016 Aug 2;16(5):1195-1203
A Short CEP135 Splice Isoform Controls Centriole Duplication.
Dahl KD, Sankaran DG, Bayless BA, Pinter ME, Galati DF, Heasley LR, Giddings TH Jr, Pearson CG
Current biology : CB 2015 Oct 5;25(19):2591-6
Current biology : CB 2015 Oct 5;25(19):2591-6
De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly.
Wang WJ, Acehan D, Kao CH, Jane WN, Uryu K, Tsou MF
eLife 2015 Nov 26;4
eLife 2015 Nov 26;4
MDM1 is a microtubule-binding protein that negatively regulates centriole duplication.
Van de Mark D, Kong D, Loncarek J, Stearns T
Molecular biology of the cell 2015 Nov 1;26(21):3788-802
Molecular biology of the cell 2015 Nov 1;26(21):3788-802
Centrobin-mediated regulation of the centrosomal protein 4.1-associated protein (CPAP) level limits centriole length during elongation stage.
Gudi R, Haycraft CJ, Bell PD, Li Z, Vasu C
The Journal of biological chemistry 2015 Mar 13;290(11):6890-902
The Journal of biological chemistry 2015 Mar 13;290(11):6890-902
Cenpj/CPAP regulates progenitor divisions and neuronal migration in the cerebral cortex downstream of Ascl1.
Garcez PP, Diaz-Alonso J, Crespo-Enriquez I, Castro D, Bell D, Guillemot F
Nature communications 2015 Mar 10;6:6474
Nature communications 2015 Mar 10;6:6474
Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4.
Wong YL, Anzola JV, Davis RL, Yoon M, Motamedi A, Kroll A, Seo CP, Hsia JE, Kim SK, Mitchell JW, Mitchell BJ, Desai A, Gahman TC, Shiau AK, Oegema K
Science (New York, N.Y.) 2015 Jun 5;348(6239):1155-60
Science (New York, N.Y.) 2015 Jun 5;348(6239):1155-60
RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity.
Shiratsuchi G, Takaoka K, Ashikawa T, Hamada H, Kitagawa D
The EMBO journal 2015 Jan 2;34(1):97-114
The EMBO journal 2015 Jan 2;34(1):97-114
Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation.
Shukla A, Kong D, Sharma M, Magidson V, Loncarek J
Nature communications 2015 Aug 21;6:8077
Nature communications 2015 Aug 21;6:8077
Centrobin-centrosomal protein 4.1-associated protein (CPAP) interaction promotes CPAP localization to the centrioles during centriole duplication.
Gudi R, Zou C, Dhar J, Gao Q, Vasu C
The Journal of biological chemistry 2014 May 30;289(22):15166-78
The Journal of biological chemistry 2014 May 30;289(22):15166-78
PIPKIγ targets to the centrosome and restrains centriole duplication.
Xu Q, Zhang Y, Xiong X, Huang Y, Salisbury JL, Hu J, Ling K
Journal of cell science 2014 Mar 15;127(Pt 6):1293-305
Journal of cell science 2014 Mar 15;127(Pt 6):1293-305
SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication.
Fong CS, Kim M, Yang TT, Liao JC, Tsou MF
Developmental cell 2014 Jul 28;30(2):238-45
Developmental cell 2014 Jul 28;30(2):238-45
Stabilization of cartwheel-less centrioles for duplication requires CEP295-mediated centriole-to-centrosome conversion.
Izquierdo D, Wang WJ, Uryu K, Tsou MF
Cell reports 2014 Aug 21;8(4):957-65
Cell reports 2014 Aug 21;8(4):957-65
A novel tankyrase small-molecule inhibitor suppresses APC mutation-driven colorectal tumor growth.
Lau T, Chan E, Callow M, Waaler J, Boggs J, Blake RA, Magnuson S, Sambrone A, Schutten M, Firestein R, Machon O, Korinek V, Choo E, Diaz D, Merchant M, Polakis P, Holsworth DD, Krauss S, Costa M
Cancer research 2013 May 15;73(10):3132-44
Cancer research 2013 May 15;73(10):3132-44
CEP120 and SPICE1 cooperate with CPAP in centriole elongation.
Comartin D, Gupta GD, Fussner E, Coyaud É, Hasegan M, Archinti M, Cheung SW, Pinchev D, Lawo S, Raught B, Bazett-Jones DP, Lüders J, Pelletier L
Current biology : CB 2013 Jul 22;23(14):1360-6
Current biology : CB 2013 Jul 22;23(14):1360-6
The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.
Zhao H, Zhu L, Zhu Y, Cao J, Li S, Huang Q, Xu T, Huang X, Yan X, Zhu X
Nature cell biology 2013 Dec;15(12):1434-44
Nature cell biology 2013 Dec;15(12):1434-44
Interaction proteomics identify NEURL4 and the HECT E3 ligase HERC2 as novel modulators of centrosome architecture.
Al-Hakim AK, Bashkurov M, Gingras AC, Durocher D, Pelletier L
Molecular & cellular proteomics : MCP 2012 Jun;11(6):M111.014233
Molecular & cellular proteomics : MCP 2012 Jun;11(6):M111.014233
Tankyrase 1 regulates centrosome function by controlling CPAP stability.
Kim MK, Dudognon C, Smith S
EMBO reports 2012 Aug;13(8):724-32
EMBO reports 2012 Aug;13(8):724-32
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse testis tissue were subjected to SDS PAGE followed by western blot with 11517-1-AP(CENPJ antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The CENPJ antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CENPJ. This antibody recognizes human,mouse,rat antigen. The CENPJ antibody has been validated for the following applications: ELISA, WB, IF analysis.
