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LearnH00055835-M02
antibody from Novus Biologicals
Targeting: CENPJ
BM032, CPAP, LAP, LIP1, MCPH6, Sas-4, SASS4, SCKL4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00055835-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055835-M02, RRID:AB_1145154
- Product name
- Mouse Monoclonal CENPJ Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CENPJ - centromere protein J (1A5)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CENPJ Antibody (1A5) [H00055835-M02] - Analysis of CENPJ expression in transfected 293T cell line by CENPJ monoclonal antibody (M02), clone 1A5.Lane 1: CENPJ transfected lysate(43.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CENPJ Antibody (1A5) [H00055835-M02] - Detection limit for recombinant GST tagged CENPJ is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CENPJ Antibody (1A5) [H00055835-M02] - Analysis of CENPJ transfected lysate using anti-CENPJ monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CENPJ MaxPab rabbit polyclonal antibody.
