RQ8312

antibody from NSJ Bioreagents

Targeting: NOP2 NOL1, NOP120, NSUN1, p120

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ8312

Provider

NSJ Bioreagents

Product name

NSUN1 Antibody / NOP2 / Nucleolar protein 2

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human

Host

Rabbit

Vial size

100 ug

Storage

After reconstitution, the Nucleolar protein 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HEL, 2) Caco-2, 3) A549, 4) HeLa, 5) RT4, 6) 293T, 7) SiHa and 8) U-251 cell lysate with Nucleolar protein 2 antibody. Predicted molecular weight: 89 kDa but routinely observed at 100~120 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with Nucleolar protein 2 antibody (red), Beta Tubulin mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human RT4 cells with Nucleolar protein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nucleolar protein 2 antibody.