Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- AHC-005-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OTOP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat kidney membranes (lanes 1 and 5), mouse heart lysate (lanes 2 and 6), mouse brain lysate (lanes 3 and 7) and rat tongue lysate (lanes 4 and 8): - 1-4. Anti-OTOP1 Antibody (#AHC-005), (1:200). 5-8. Anti-OTOP1 Antibody , preincubated with OTOP1 Blocking Peptide (#BLP-HC005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat kidney membranes (lanes 1 and 5), mouse heart lysate (lanes 2 and 6), mouse brain lysate (lanes 3 and 7) and rat tongue lysate (lanes 4 and 8): - 1-4. Anti-OTOP1 Antibody (#AHC-005), (1:200). 5-8. Anti-OTOP1 Antibody , preincubated with OTOP1 Blocking Peptide (#BLP-HC005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Otopetrin-1 in mouse hippocampus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Otopetrin-1 Antibody (#AHC-005), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA1 region, showed Otopetrin-1 immunoreactivity (green) in astrocytic profiles (arrows). B. Pre-incubation of the Antibody with Otopetrin-1 Blocking Peptide (BLP-HC005), suppressed staining. Cell nuclei are stained with DAPI (blue). P= pyramidal layer, SR = stratum radiatum.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Otopetrin-1 in rat cerebellum. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Otopetrin-1 Antibody (#AHC-005), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Otopetrin-1 immunoreactivity (green) appeared in Bergmann glial profiles (arrows). B. Pre-incubation of the Antibody with Otopetrin-1 Blocking Peptide (BLP-HC005), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer. P= purkinje layer.