PAB20512

antibody from Abnova Corporation

Targeting: B4GALT1 beta4Gal-T1, GGTB2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20512

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20512, RRID:AB_10963195

Product name

B4GALT1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant B4GALT1.

Antigen sequence

LPQLVGVSTPLQGGSNSAAAIGQSSGELRTGGARP
PPPLGASSQPRPGGDSSPVVDSGPGPASNLTSVPV
PHTTALSLPACPEESPLLVGPMLIEFNMPVDLELV
AKQNPNVKM


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with B4GALT1 polyclonal antibody (Cat # PAB20512) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with B4GALT1 polyclonal antibody (Cat # PAB20512) at 1-4 ug/mL dilution shows positivity in golgi apparatus.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate with B4GALT1 polyclonal antibody (Cat # PAB20512) shows strong granular cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)