STJ111285

antibody from St John's Laboratory

Targeting: B4GALT1 beta4Gal-T1, GGTB2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

STJ111285

Provider

St John's Laboratory

Product name

Anti-B4GALT1 antibody (50-215) (STJ111285)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody anti-B4GALT1 (50-215) is suitable for use in Western Blot and Immunohistochemistry.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NA


Epitope

NA

Isotype

IgG

Antibody clone number

NA

Vial size

NA

Concentration

NA

Storage

Store in a freezer at-20°C and avoid freeze-thaw cycles.

Handling

NA

Western blot

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using B4GALT1 antibody (STJ111285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

Show more

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Western blot analysis of extracts from normal (control) and B4GALT1 knockout (KO) HeLa cells, using B4GALT1 antibody (STJ111285) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

Show more