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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-803-339-C050 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-803-339-C050, RRID:AB_11181209
- Product name
- B4GALT1 (human) monoclonal antibody (GT2/36/118)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Protein G-affinity purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT2/36/118
- Vial size
- 50 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references Golgi function and dysfunction in the first COG4-deficient CDG type II patient.
Clathrin-dependent association of CVAK104 with endosomes and the trans-Golgi network.
Golgi inheritance in mammalian cells is mediated through endoplasmic reticulum export activities.
Tumor necrosis factor-alpha up-regulates the expression of beta1,4-galactosyltransferase I in primary human endothelial cells by mRNA stabilization.
The palmitoyltransferase of the cation-dependent mannose 6-phosphate receptor cycles between the plasma membrane and endosomes.
SseG, a virulence protein that targets Salmonella to the Golgi network.
Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network.
Dynamics of transitional endoplasmic reticulum sites in vertebrate cells.
Endoplasmic reticulum-to-cytosol transport of free polymannose oligosaccharides in permeabilized HepG2 cells.
Kin recognition between medial Golgi enzymes in HeLa cells.
The TGN38 glycoprotein contains two non-overlapping signals that mediate localization to the trans-Golgi network.
Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa.
Monoclonal antibodies to soluble, human milk galactosyltransferase (lactose synthase A protein).
Reynders E, Foulquier F, Leão Teles E, Quelhas D, Morelle W, Rabouille C, Annaert W, Matthijs G
Human molecular genetics 2009 Sep 1;18(17):3244-56
Human molecular genetics 2009 Sep 1;18(17):3244-56
Clathrin-dependent association of CVAK104 with endosomes and the trans-Golgi network.
Düwel M, Ungewickell EJ
Molecular biology of the cell 2006 Oct;17(10):4513-25
Molecular biology of the cell 2006 Oct;17(10):4513-25
Golgi inheritance in mammalian cells is mediated through endoplasmic reticulum export activities.
Altan-Bonnet N, Sougrat R, Liu W, Snapp EL, Ward T, Lippincott-Schwartz J
Molecular biology of the cell 2006 Feb;17(2):990-1005
Molecular biology of the cell 2006 Feb;17(2):990-1005
Tumor necrosis factor-alpha up-regulates the expression of beta1,4-galactosyltransferase I in primary human endothelial cells by mRNA stabilization.
García-Vallejo JJ, van Dijk W, van Die I, Gringhuis SI
The Journal of biological chemistry 2005 Apr 1;280(13):12676-82
The Journal of biological chemistry 2005 Apr 1;280(13):12676-82
The palmitoyltransferase of the cation-dependent mannose 6-phosphate receptor cycles between the plasma membrane and endosomes.
Stöckli J, Rohrer J
Molecular biology of the cell 2004 Jun;15(6):2617-26
Molecular biology of the cell 2004 Jun;15(6):2617-26
SseG, a virulence protein that targets Salmonella to the Golgi network.
Salcedo SP, Holden DW
The EMBO journal 2003 Oct 1;22(19):5003-14
The EMBO journal 2003 Oct 1;22(19):5003-14
Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network.
Rohrer J, Kornfeld R
Molecular biology of the cell 2001 Jun;12(6):1623-31
Molecular biology of the cell 2001 Jun;12(6):1623-31
Dynamics of transitional endoplasmic reticulum sites in vertebrate cells.
Hammond AT, Glick BS
Molecular biology of the cell 2000 Sep;11(9):3013-30
Molecular biology of the cell 2000 Sep;11(9):3013-30
Endoplasmic reticulum-to-cytosol transport of free polymannose oligosaccharides in permeabilized HepG2 cells.
Moore SE, Bauvy C, Codogno P
The EMBO journal 1995 Dec 1;14(23):6034-42
The EMBO journal 1995 Dec 1;14(23):6034-42
Kin recognition between medial Golgi enzymes in HeLa cells.
Nilsson T, Hoe MH, Slusarewicz P, Rabouille C, Watson R, Hunte F, Watzele G, Berger EG, Warren G
The EMBO journal 1994 Feb 1;13(3):562-74
The EMBO journal 1994 Feb 1;13(3):562-74
The TGN38 glycoprotein contains two non-overlapping signals that mediate localization to the trans-Golgi network.
Ponnambalam S, Rabouille C, Luzio JP, Nilsson T, Warren G
The Journal of cell biology 1994 Apr;125(2):253-68
The Journal of cell biology 1994 Apr;125(2):253-68
Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa.
Linstedt AD, Hauri HP
Molecular biology of the cell 1993 Jul;4(7):679-93
Molecular biology of the cell 1993 Jul;4(7):679-93
Monoclonal antibodies to soluble, human milk galactosyltransferase (lactose synthase A protein).
Berger EG, Aegerter E, Mandel T, Hauri HP
Carbohydrate research 1986 Jun 1;149(1):23-33
Carbohydrate research 1986 Jun 1;149(1):23-33
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunocytochemistry using B4GALT1, mAb (GT2/36/118) (Prod. No. ALX-803-339). The specificity of the monoclonal antibody was confirmed by immunofluorescence staining of the Golgi apparatus in HeLa cells. Method: B4GALT1, mAb (GT2/36/118) was diluted 1:500 and applied to saponin-permeabilized HeLa cells (after fixation in 3% paraformadehyde). A goat-anti-mouse Alexa488 antibody was used as secondary antibody. DAPI was used to stain the nuclei. The Golgi apparatus shows as a typical perinuclear structure.
