Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 12093-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12093-1-AP, RRID:AB_2211743
- Product name
- UBA5 antibody
- Antibody type
- Polyclonal
- Description
- UBA5 antibody (Cat. #12093-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis.
Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.
Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway.
Eck F, Phuyal S, Smith MD, Kaulich M, Wilkinson S, Farhan H, Behrends C
Journal of cell science 2020 Sep 16;133(18)
Journal of cell science 2020 Sep 16;133(18)
Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.
Colin E, Daniel J, Ziegler A, Wakim J, Scrivo A, Haack TB, Khiati S, Denommé AS, Amati-Bonneau P, Charif M, Procaccio V, Reynier P, Aleck KA, Botto LD, Herper CL, Kaiser CS, Nabbout R, N'Guyen S, Mora-Lorca JA, Assmann B, Christ S, Meitinger T, Strom TM, Prokisch H, FREX Consortium., Miranda-Vizuete A, Hoffmann GF, Lenaers G, Bomont P, Liebau E, Bonneau D
American journal of human genetics 2016 Sep 1;99(3):695-703
American journal of human genetics 2016 Sep 1;99(3):695-703
Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway.
Gavin JM, Hoar K, Xu Q, Ma J, Lin Y, Chen J, Chen W, Bruzzese FJ, Harrison S, Mallender WD, Bump NJ, Sintchak MD, Bence NF, Li P, Dick LR, Gould AE, Chen JJ
The Journal of biological chemistry 2014 Aug 15;289(33):22648-22658
The Journal of biological chemistry 2014 Aug 15;289(33):22648-22658
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12093-1-AP(UBA5 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using UBA5 antibody 12093-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-UBA5 (IP:12093-1-AP, 4ug; Detection:12093-1-AP 1:1000) with HeLa cells lysate 1800ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 12093-1-AP(UBA5 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue