Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12093-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12093-1-AP, RRID:AB_2211743
- Product name
- UBA5 antibody
- Antibody type
- Polyclonal
- Description
- UBA5 antibody (Cat. #12093-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The UFMylation pathway is impaired in Alzheimer's disease.
UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER.
UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity.
Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice.
ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis.
Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.
Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway.
Yan T, Heckman MG, Craver EC, Liu CC, Rawlinson BD, Wang X, Murray ME, Dickson DW, Ertekin-Taner N, Lou Z, Bu G, Springer W, Fiesel FC
bioRxiv : the preprint server for biology 2024 May 26;
bioRxiv : the preprint server for biology 2024 May 26;
UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER.
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wąchalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature 2024 Mar;627(8003):445-452
Nature 2024 Mar;627(8003):445-452
UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity.
He C, Xing X, Chen HY, Gao M, Shi J, Xiang B, Xiao X, Sun Y, Yu H, Xu G, Yao Y, Xie Z, Xing Y, Budiarto BR, Chen SY, Gao Y, Lee YR, Zhang J
Molecular cell 2024 Mar 21;84(6):1120-1138.e8
Molecular cell 2024 Mar 21;84(6):1120-1138.e8
Screening UFMylation-associated genes in heart tissues of Ufm1-transgenic mice.
Jiajia H, Ziyao Y, Jiaqi Z, Yanli C, Xiaotao Z, Ming S
BMC cardiovascular disorders 2023 Nov 18;23(1):567
BMC cardiovascular disorders 2023 Nov 18;23(1):567
ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis.
Eck F, Phuyal S, Smith MD, Kaulich M, Wilkinson S, Farhan H, Behrends C
Journal of cell science 2020 Sep 16;133(18)
Journal of cell science 2020 Sep 16;133(18)
Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.
Colin E, Daniel J, Ziegler A, Wakim J, Scrivo A, Haack TB, Khiati S, Denommé AS, Amati-Bonneau P, Charif M, Procaccio V, Reynier P, Aleck KA, Botto LD, Herper CL, Kaiser CS, Nabbout R, N'Guyen S, Mora-Lorca JA, Assmann B, Christ S, Meitinger T, Strom TM, Prokisch H, FREX Consortium, Miranda-Vizuete A, Hoffmann GF, Lenaers G, Bomont P, Liebau E, Bonneau D
American journal of human genetics 2016 Sep 1;99(3):695-703
American journal of human genetics 2016 Sep 1;99(3):695-703
Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway.
Gavin JM, Hoar K, Xu Q, Ma J, Lin Y, Chen J, Chen W, Bruzzese FJ, Harrison S, Mallender WD, Bump NJ, Sintchak MD, Bence NF, Li P, Dick LR, Gould AE, Chen JJ
The Journal of biological chemistry 2014 Aug 15;289(33):22648-22658
The Journal of biological chemistry 2014 Aug 15;289(33):22648-22658
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12093-1-AP(UBA5 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using UBA5 antibody 12093-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-UBA5 (IP:12093-1-AP, 4ug; Detection:12093-1-AP 1:1000) with HeLa cells lysate 1800ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 12093-1-AP(UBA5 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue