PA5-120577

antibody from Invitrogen Antibodies

Targeting: PCDHB4 PCDH-BETA4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120577

Provider

Invitrogen Antibodies

Product name

PCDHB4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: 293T, Raji, Mouse testis, Rat testis. Immunogen sequence: EPIRYSVLEE TESGSFVAHL AKDLGLGIGE LASRSARVLS DDDKQRLQLD RQTGDLLLRE KLDREELCGP IEPCVLHFQV FLEMPVQFFQ GELLIQDIND HSPIFPEREV LLKILENSQP GTLFPLLIAE DLDVGSNGLQ KYTISPNSHF HILTRNHSEG KKYPDLVQDK PLDREEQPEF SLTLVALDGG SPPRSGTVMV RILIMDINDN APEFVHTPYG VQVLENSPLD SPIVRVLARD IDAGNFGSVS YGLFQASDEI KQTFSINEVT GEI

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PCDHB4 in various cell lines using a PCDHB4 Polyclonal antibody (Product # PA5-120577) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 5s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of PCDHB4 in L929 cells using a PCDHB4 Polyclonal antibody (Product # PA5-120577). Blue: DAPI for nuclear staining.