Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 20461-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EPS8L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSLWESLGE SWMRPRSEWP REPQVPLYVP KFHSGWEPPV DVLQEAPWEV EGLASAPIEE VSPVSRQSIR NSQKHSPTSE PTPPGDALPP VSSPHTHRGC QPTPAMAKYV KILYDFTARN ANELSVLKDE VLEVLEDGRQ WWKLRSRSGQ AGYVPCNILG EARPEDAGAP FEQAGQKYWG PASPTHKLPP SFPGNKDELM QHMDEVNDEL IRKISNIRAQ PQRHFRVERS QPVSQPLTYE SGPDEVRAWL EAKAFSPRIV ENLGILTGPQ LFSLNKEELK KVCGEEGVRV YSQLTMQKAF LEKQQSGSEL EELMNKFHSM NQRRGEDS (389-715 aa encoded by BC002474)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 20461-1-AP (EPS8L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 20461-1-AP (EPS8L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 20461-1-AP (EPS8L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skin tissue were subjected to SDS PAGE followed by western blot with 20461-1-AP (EPS8L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 20461-1-AP (EPS8L2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 20461-1-AP (EPS8L2 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 20461-1-AP (EPS8L2 antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-EPS8L2 (IP:20461-1-AP, 4ug; Detection:20461-1-AP 1:1000) with HeLa cells lysate 3200ug.
