ARG65748

antibody from Arigo

Targeting: PDIA2 PDA2, PDI, PDIP, PDIR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG65748

Provider

Arigo

Product name

anti-PDI antibody

Antibody type

Polyclonal

Antigen

KLH-conjugated synthetic peptide corresponding to a region of Human PDI (NP_006840.2).

Description

Affinity purification with immunogen.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 30 µg of 1) MCF-7, 2) Mouse liver, and 3) MEF lysates stained with ARG65748 anti-PDI antibody at 1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunocytochemistry: HUVEC cells stained with ARG65748 anti-PDI antibody (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunocytochemistry: A549 cells stained with ARG65748 anti-PDI antibody (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunocytochemistry: NIH/3T3 cells stained with ARG65748 anti-PDI antibody (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: paraffin-embedded Human lung carcinoma tissue stained with ARG65748 anti-PDI antibody. Counter stained with hematoxylin.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: paraffin-embedded Human colon carcinoma tissue stained with ARG65748 anti-PDI antibody. Counter stained with hematoxylin.