UM500025
antibody from Invitrogen Antibodies
Targeting: FOLH1
FOLH, GCP2, GCPII, NAALAD1, NAALAdase, PSM, PSMA
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [1]
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Validation data
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- Product number
- UM500025 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOLH1 Monoclonal Antibody (UMAB25), UltraMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB25
- Vial size
- 100 µL
- Concentration
- 0.5-1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35 µg) from MDCK. Dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of 10 different human tissue lysates (10 µg) by using anti-FOLH1 monoclonal antibody (clone UMAB25, 1:500)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of mouse tissue lysates (20 µg) from Brain. Dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PC-12 cells using FOLH1 mouse monoclonal antibody (UM500025, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FOLH1 mouse monoclonal antibody (Clone UMAB25). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-FOLH1 (Clone UMAB25) very specifically recognizes FOLH1 antigen on OriGene protein microarray chip.