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- Antibody Data
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- References [12]
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- Product number
- MAB177-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17RA/IL-17R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-17 RA/IL-17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-17 R, rmIL-17B R, or recombinant human IL-17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q96F46- Isotype
- IgG
- Antibody clone number
- 133617
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Autophagy impacts on oxaliplatin-induced hepatocarcinoma apoptosis via the IL-17/IL-17R-JAK2/STAT3 signaling pathway.
Rapid and Rigorous IL-17A Production by a Distinct Subpopulation of Effector Memory T Lymphocytes Constitutes a Novel Mechanism of Toxic Shock Syndrome Immunopathology.
Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma.
Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
IL-17 induces monocyte migration in rheumatoid arthritis.
IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood.
CD molecules 2006--human cell differentiation molecules.
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Wu J, Guo J, Cao Q, Wang Y, Chen J, Wang Z, Yuan Z
Oncology letters 2017 Feb;13(2):770-776
Oncology letters 2017 Feb;13(2):770-776
Rapid and Rigorous IL-17A Production by a Distinct Subpopulation of Effector Memory T Lymphocytes Constitutes a Novel Mechanism of Toxic Shock Syndrome Immunopathology.
Szabo PA, Goswami A, Mazzuca DM, Kim K, O'Gorman DB, Hess DA, Welch ID, Young HA, Singh B, McCormick JK, Haeryfar SM
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2805-2818
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2805-2818
Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
European journal of immunology 2016 Feb;46(2):440-5
European journal of immunology 2016 Feb;46(2):440-5
Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
Mucosal immunology 2012 Mar;5(2):140-9
Mucosal immunology 2012 Mar;5(2):140-9
IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
Ramirez-Carrozzi V, Sambandam A, Luis E, Lin Z, Jeet S, Lesch J, Hackney J, Kim J, Zhou M, Lai J, Modrusan Z, Sai T, Lee W, Xu M, Caplazi P, Diehl L, de Voss J, Balazs M, Gonzalez L Jr, Singh H, Ouyang W, Pappu R
Nature immunology 2011 Oct 12;12(12):1159-66
Nature immunology 2011 Oct 12;12(12):1159-66
T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma.
Corrigan CJ, Wang W, Meng Q, Fang C, Wu H, Reay V, Lv Z, Fan Y, An Y, Wang YH, Liu YJ, Lee TH, Ying S
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 25;108(4):1579-84
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 25;108(4):1579-84
Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
International journal of cancer 2009 Sep 1;125(5):1092-101
International journal of cancer 2009 Sep 1;125(5):1092-101
Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
Circulation 2009 Mar 17;119(10):1424-32
Circulation 2009 Mar 17;119(10):1424-32
IL-17 induces monocyte migration in rheumatoid arthritis.
Shahrara S, Pickens SR, Dorfleutner A, Pope RM
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3884-91
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3884-91
IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood.
Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P
Journal of immunology (Baltimore, Md. : 1950) 2008 Jan 1;180(1):655-63
Journal of immunology (Baltimore, Md. : 1950) 2008 Jan 1;180(1):655-63
CD molecules 2006--human cell differentiation molecules.
Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Bühring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
Journal of immunological methods 2007 Jan 30;319(1-2):1-5
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-17 RA/IL-17 R Specificity is Shown by Flow Cytometry in Knockout Cell Line. IL-17 RA/IL-17 R knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the IL-17 RA/IL-17 R knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL1/GRO alpha Secretion Induced by IL-17/IL-17A and Neutralization by Human IL-17 RA/IL-17 R Antibody. Recombinant Human IL-17/IL-17A induces CXCL1/GRO alpha secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha DuoSet (Catalog # DY275). CXCL1/GRO alpha secretion elicited by Recombinant Human IL-17/IL-17A (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177). The ND50 is typically 0.4-2 μg/mL.
