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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- AM09377PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09377PU-N, RRID:AB_10755050
- Product name
- anti Axin-1
- Antibody type
- Monoclonal
- Antigen
- Recombinant human Axin1 (546-752aa) purified from E. coli
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT1A4
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Axin1 has 44% homology with Axin2 (Conductin) and its function is similar, but they have different expression patterns. Axin1 is widely expressed and detected at a very low level, whereas Axin2 is differentially expressed in various cells and tissue (stomach cancer or colorectal cancer). Also, it is reported that Axin2 was highly over-expressed in AGS and SW480 cell lines. Immunoprecipitation (IP) and western blot experiments were performed to evaluate Axin1 antibody's specificity. IP sample and cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-mouse Axin1 (1:500). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis:Axin1 has 44% homology with Axin2 (Conductin) and its function is similar, but they have different expression patterns. Axin1 is widely expressed and detected at a very low level, whereas Axin2 is differentially expressed in various cells and tissue (stomach cancer or colorectal cancer). Also, it is reported that Axin2 was highly over-expressed in AGS and SW480 cell lines. Immunoprecipitation (IP) and western blot experiments were performed to evaluate Axin1 antibodyâs specificity. IP sample and cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-mouse Axin1 (1:500). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of stained human HeLa cells was done with Hoechst 33342 (Blue) for chromatin staining and Texas Red (Red) for monoclonal anti-human Axin1a antibody (1:500).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of stained human HeLa cells was done with Hoechst 33342 (Blue) for chromatin staining and Texas Red (Red) for monoclonal anti-human Axin1a antibody (1:500).
