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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB10012 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10012, RRID:AB_10903406
- Product name
- AXIN1 monoclonal antibody, clone AT1A4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant AXIN1.
- Isotype
- IgG
- Antibody clone number
- AT1A4
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation (Lane 1 and 2) and Western blot (Lane 3, 4 and 5) analysis in various cells and tissue extracts with AXIN1 monoclonal antibody, clone AT1A4 (Cat # MAB10012) at 1:500 dilution.Lane 1, normal IgG.Lane 2, HeLa.Lane 3, HeLa.Lane 4, AGS.Lane 5, SW480.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human HeLa cells was done with Hoechst 33342 (Blue) for chromatin staining and Texas Red (Red) with AXIN1 monoclonal antibody, clone AT1A4 (Cat # MAB10012) at 1:500 dilution.
- Validation comment
- Immunofluorescence
