CABT-RM107

antibody from Creative Diagnostics

Targeting: PRDM9 KMT8B, MSBP3, PFM6, ZNF899

Western blot (Recommended by provider)

Antibody Data

Product number

CABT-RM107

Provider

Creative Diagnostics

Product name

Rabbit Anti-PRDM9 polyclonal antibody

Antibody type

Polyclonal

Description

Histone-lysine N-methyltransferase PRDM9 is encoded by PRDM9 gene in human. PRDM9 is a zinc-finger protein that catalyzes histone H3 Lysine 4 trimethylation (H3K4me3), but lacks capacity to mono- and dimethylate Lysine 4 of histone H3. H3 Lysine -4 methylation plays a vital role in the transcriptional activation of genes during early meiotic prophase. PRDM9 contains multiple domains, including a Kruppel-associated box (KRAB) domain (aa 23-86), an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain (aa 244-358), and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. PRDM9 is reported to be highly polymorphic, both within and between mammalian species. It is shown that most of the naturally occurring sequence polymorphisms in PRDM9 change the identity of the amino acids contacting DNA and/or the number and arrangement of individual fingers in the DNA-binding zinc-finger domains. This allows PRDM9 variants to target a large number of DNA sequences, thereby expanding the distribution of recombination sites.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

25 _g

Storage

Stable for 1 year at 2-8°C from date of receipt.