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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504610 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504610, RRID:AB_11125377
- Product name
- FXYD3 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)
- Antibody type
- Monoclonal
- Description
- FXYD3 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FXYD3
- Isotype
- IgG
- Antibody clone number
- OTI1D1
- Vial size
- 100 µl
- Concentration
- 0.74 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FXYD3 (RC213945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FXYD3.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 1 cell line by using anti-FXYD3 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-FXYD3 mouse monoclonal antibody (TA504610) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FXYD3(RC213945).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-FXYD3 antibody(TA504610),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
