15853-1-AP

antibody from Invitrogen Antibodies

Targeting: FXYD3 MAT-8, PLML

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15853-1-AP

Provider

Invitrogen Antibodies

Product name

FXYD3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MQKVTLGLL VFLAGFPVLD ANDLEDKNSP FYYDWHSLQV GGLICAGVLC AMGIIIVMSA KCKCKFGQKS GHHPGETPPL ITPGSAQS (1-87 aa encoded by BC005238)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 15853-1-AP (FXYD3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15853-1-AP (FXYD3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 15853-1-AP (FXYD3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-FXYD3 (IP:15853-1-AP, 4ug; Detection:15853-1-AP 1:500) with COLO 320 cells lysate 2400ug.