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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-97595 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-97595, RRID:AB_11190087
- Product name
- Mouse Monoclonal LRDD Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references An IRAK1-PIN1 signalling axis drives intrinsic tumour resistance to radiation therapy.
Identification of TRIML2, a novel p53 target, that enhances p53 SUMOylation and regulates the transactivation of proapoptotic genes.
Autoproteolysis of PIDD marks the bifurcation between pro-death caspase-2 and pro-survival NF-kappaB pathway.
Liu PH, Shah RB, Li Y, Arora A, Ung PM, Raman R, Gorbatenko A, Kozono S, Zhou XZ, Brechin V, Barbaro JM, Thompson R, White RM, Aguirre-Ghiso JA, Heymach JV, Lu KP, Silva JM, Panageas KS, Schlessinger A, Maki RG, Skinner HD, de Stanchina E, Sidi S
Nature cell biology 2019 Feb;21(2):203-213
Nature cell biology 2019 Feb;21(2):203-213
Identification of TRIML2, a novel p53 target, that enhances p53 SUMOylation and regulates the transactivation of proapoptotic genes.
Kung CP, Khaku S, Jennis M, Zhou Y, Murphy ME
Molecular cancer research : MCR 2015 Feb;13(2):250-62
Molecular cancer research : MCR 2015 Feb;13(2):250-62
Autoproteolysis of PIDD marks the bifurcation between pro-death caspase-2 and pro-survival NF-kappaB pathway.
Tinel A, Janssens S, Lippens S, Cuenin S, Logette E, Jaccard B, Quadroni M, Tschopp J
The EMBO journal 2007 Jan 10;26(1):197-208
The EMBO journal 2007 Jan 10;26(1):197-208
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LRDD Antibody (Anto-1) [NBP1-97595] - Analysis of endogenous and overexpressed human PIDD using MAb to PIDD (Anto-1). Method: Cell extracts (1x10^5) from HEK 293T wild type (lane 1) or transfected with a plasmid coding for human PIDD (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to PIDD (Anto-1) at a 1:500 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. Note that two major forms of PIDD are detected, representing full length and cleaved proteins, migrating at approximatively 90kDa and 50kDa, respectively.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LRDD Antibody (Anto-1) [NBP1-97595] - Staining of endogenous human PIDD in different human tissues (paraffin sections) using MAb to PIDD (Anto-1). Method: Different human normal (A: Bronchus; B: Kidney; C: Lung) or cancer (D: Breast; E: Ovarian; F: Colo-rectal) tissues were stained with MAb to PIDD (Anto-1) by standard immunohistochemistry.
