Antibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 14927-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14927-1-AP, RRID:AB_11183050
- Product name
- TIM22 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated TIM22 antibody (Cat. #14927-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Cytosolic retention of HtrA2 during mitochondrial protein import stress triggers the DELE1-HRI pathway.
TIM22 and TIM29 inhibit HBV replication by up-regulating SRSF1 expression.
The SARS-CoV-2 protein ORF3c is a mitochondrial modulator of innate immunity.
NOX4-TIM23 interaction regulates NOX4 mitochondrial import and metabolic reprogramming.
ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing.
Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria.
Pomegranate Extract (POMx) Induces Mitochondrial Dysfunction and Apoptosis of Oral Cancer Cells.
Defining the architecture of the human TIM22 complex by chemical crosslinking.
Identification and detection of mecciRNAs.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Long-term exposure to environmental level of phenanthrene causes adaptive immune response and fibrosis in mouse kidneys.
Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.
Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.
Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.
The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.
MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.
Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7.
Mitochondrial carrier protein overloading and misfolding induce aggresomes and proteostatic adaptations in the cytosol.
APOL1 Kidney Risk Variants Induce Cell Death via Mitochondrial Translocation and Opening of the Mitochondrial Permeability Transition Pore.
Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8(+) T Cells.
A Hexokinase 2 Modulator for Field-Directed Treatment of Experimental Actinic Keratoses.
Mutations of the mitochondrial carrier translocase channel subunit TIM22 cause early-onset mitochondrial myopathy.
Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis.
Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.
The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly.
TIM29 is a subunit of the human carrier translocase required for protein transport.
The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.
Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.
DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.
Bi PY, Killackey SA, Schweizer L, Arnoult D, Philpott DJ, Girardin SE
Communications biology 2024 Mar 30;7(1):391
Communications biology 2024 Mar 30;7(1):391
TIM22 and TIM29 inhibit HBV replication by up-regulating SRSF1 expression.
Guo L, Liu JJ, Long SY, Wang PY, Li S, Wang JL, Wei XF, Li J, Lei L, Huang AL, Hu JL
Journal of medical virology 2024 Feb;96(2):e29439
Journal of medical virology 2024 Feb;96(2):e29439
The SARS-CoV-2 protein ORF3c is a mitochondrial modulator of innate immunity.
Stewart H, Lu Y, O'Keefe S, Valpadashi A, Cruz-Zaragoza LD, Michel HA, Nguyen SK, Carnell GW, Lukhovitskaya N, Milligan R, Adewusi Y, Jungreis I, Lulla V, Matthews DA, High S, Rehling P, Emmott E, Heeney JL, Davidson AD, Edgar JR, Smith GL, Firth AE
iScience 2023 Nov 17;26(11):108080
iScience 2023 Nov 17;26(11):108080
NOX4-TIM23 interaction regulates NOX4 mitochondrial import and metabolic reprogramming.
Pandey J, Larson-Casey JL, Patil MH, Joshi R, Jiang CS, Zhou Y, He C, Carter AB
The Journal of biological chemistry 2023 May;299(5):104695
The Journal of biological chemistry 2023 May;299(5):104695
ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing.
Clemente P, Calvo-Garrido J, Pearce SF, Schober FA, Shigematsu M, Siira SJ, Laine I, Spåhr H, Steinmetzger C, Petzold K, Kirino Y, Wibom R, Rackham O, Filipovska A, Rorbach J, Freyer C, Wredenberg A
Nature communications 2022 Sep 30;13(1):5750
Nature communications 2022 Sep 30;13(1):5750
Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria.
Chojnacka KJ, Elancheliyan P, Mussulini BHM, Mohanraj K, Callegari S, Gosk A, Banach T, Góral T, Szczepanowska K, Rehling P, Serwa RA, Chacinska A
Molecular biology of the cell 2022 Apr 1;33(4):ar29
Molecular biology of the cell 2022 Apr 1;33(4):ar29
Pomegranate Extract (POMx) Induces Mitochondrial Dysfunction and Apoptosis of Oral Cancer Cells.
Peng SY, Lin LC, Chen SR, Farooqi AA, Cheng YB, Tang JY, Chang HW
Antioxidants (Basel, Switzerland) 2021 Jul 13;10(7)
Antioxidants (Basel, Switzerland) 2021 Jul 13;10(7)
Defining the architecture of the human TIM22 complex by chemical crosslinking.
Valpadashi A, Callegari S, Linden A, Neumann P, Ficner R, Urlaub H, Deckers M, Rehling P
FEBS letters 2021 Jan;595(2):157-168
FEBS letters 2021 Jan;595(2):157-168
Identification and detection of mecciRNAs.
Liu X, Yang Y, Shan G
Methods (San Diego, Calif.) 2021 Dec;196:147-152
Methods (San Diego, Calif.) 2021 Dec;196:147-152
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Morgenstern M, Peikert CD, Lübbert P, Suppanz I, Klemm C, Alka O, Steiert C, Naumenko N, Schendzielorz A, Melchionda L, Mühlhäuser WWD, Knapp B, Busch JD, Stiller SB, Dannenmaier S, Lindau C, Licheva M, Eickhorst C, Galbusera R, Zerbes RM, Ryan MT, Kraft C, Kozjak-Pavlovic V, Drepper F, Dennerlein S, Oeljeklaus S, Pfanner N, Wiedemann N, Warscheid B
Cell metabolism 2021 Dec 7;33(12):2464-2483.e18
Cell metabolism 2021 Dec 7;33(12):2464-2483.e18
Long-term exposure to environmental level of phenanthrene causes adaptive immune response and fibrosis in mouse kidneys.
Ruan F, Wu L, Yin H, Fang L, Tang C, Huang S, Fang L, Zuo Z, He C, Huang J
Environmental pollution (Barking, Essex : 1987) 2021 Aug 15;283:117028
Environmental pollution (Barking, Essex : 1987) 2021 Aug 15;283:117028
Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.
Wei X, Du M, Xie J, Luo T, Zhou Y, Zhang K, Li J, Chen D, Xu P, Jia M, Zhou H, Fang H, Lyu J, Yang Y
Journal of human genetics 2020 Mar;65(3):231-240
Journal of human genetics 2020 Mar;65(3):231-240
Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.
Gomkale R, Cruz-Zaragoza LD, Suppanz I, Guiard B, Montoya J, Callegari S, Pacheu-Grau D, Warscheid B, Rehling P
Current biology : CB 2020 Mar 23;30(6):1119-1127.e5
Current biology : CB 2020 Mar 23;30(6):1119-1127.e5
Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.
Singh RP, Jeyaraju DV, Voisin V, Hurren R, Xu C, Hawley JR, Barghout SH, Khan DH, Gronda M, Wang X, Jitkova Y, Sharon D, Liyanagae S, MacLean N, Seneviratene AK, Mirali S, Borenstein A, Thomas GE, Soriano J, Orouji E, Minden MD, Arruda A, Chan SM, Bader GD, Lupien M, Schimmer AD
Cell stem cell 2020 Jun 4;26(6):926-937.e10
Cell stem cell 2020 Jun 4;26(6):926-937.e10
The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.
Hoshino A, Wang WJ, Wada S, McDermott-Roe C, Evans CS, Gosis B, Morley MP, Rathi KS, Li J, Li K, Yang S, McManus MJ, Bowman C, Potluri P, Levin M, Damrauer S, Wallace DC, Holzbaur ELF, Arany Z
Nature 2019 Nov;575(7782):375-379
Nature 2019 Nov;575(7782):375-379
MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.
Busch JD, Cipullo M, Atanassov I, Bratic A, Silva Ramos E, Schöndorf T, Li X, Pearce SF, Milenkovic D, Rorbach J, Larsson NG
Cell reports 2019 Nov 5;29(6):1728-1738.e9
Cell reports 2019 Nov 5;29(6):1728-1738.e9
Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7.
Mohanraj K, Wasilewski M, Benincá C, Cysewski D, Poznanski J, Sakowska P, Bugajska Z, Deckers M, Dennerlein S, Fernandez-Vizarra E, Rehling P, Dadlez M, Zeviani M, Chacinska A
EMBO molecular medicine 2019 May;11(5)
EMBO molecular medicine 2019 May;11(5)
Mitochondrial carrier protein overloading and misfolding induce aggresomes and proteostatic adaptations in the cytosol.
Liu Y, Wang X, Coyne LP, Yang Y, Qi Y, Middleton FA, Chen XJ
Molecular biology of the cell 2019 May 15;30(11):1272-1284
Molecular biology of the cell 2019 May 15;30(11):1272-1284
APOL1 Kidney Risk Variants Induce Cell Death via Mitochondrial Translocation and Opening of the Mitochondrial Permeability Transition Pore.
Shah SS, Lannon H, Dias L, Zhang JY, Alper SL, Pollak MR, Friedman DJ
Journal of the American Society of Nephrology : JASN 2019 Dec;30(12):2355-2368
Journal of the American Society of Nephrology : JASN 2019 Dec;30(12):2355-2368
Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8(+) T Cells.
Hu Z, Qu G, Yu X, Jiang H, Teng XL, Ding L, Hu Q, Guo X, Zhou Y, Wang F, Li HB, Chen L, Jiang J, Su B, Liu J, Zou Q
Cell metabolism 2019 Aug 6;30(2):290-302.e5
Cell metabolism 2019 Aug 6;30(2):290-302.e5
A Hexokinase 2 Modulator for Field-Directed Treatment of Experimental Actinic Keratoses.
Behar V, Pahima H, Kozminsky-Atias A, Arbel N, Loeb E, Herzberg M, Becker OM
The Journal of investigative dermatology 2018 Dec;138(12):2635-2643
The Journal of investigative dermatology 2018 Dec;138(12):2635-2643
Mutations of the mitochondrial carrier translocase channel subunit TIM22 cause early-onset mitochondrial myopathy.
Pacheu-Grau D, Callegari S, Emperador S, Thompson K, Aich A, Topol SE, Spencer EG, McFarland R, Ruiz-Pesini E, Torkamani A, Taylor RW, Montoya J, Rehling P
Human molecular genetics 2018 Dec 1;27(23):4135-4144
Human molecular genetics 2018 Dec 1;27(23):4135-4144
Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis.
Guo W, Liu W, Chen Z, Gu Y, Peng S, Shen L, Shen Y, Wang X, Feng GS, Sun Y, Xu Q
Nature communications 2017 Dec 18;8(1):2168
Nature communications 2017 Dec 18;8(1):2168
Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.
Vukotic M, Nolte H, König T, Saita S, Ananjew M, Krüger M, Tatsuta T, Langer T
Molecular cell 2017 Aug 3;67(3):471-483.e7
Molecular cell 2017 Aug 3;67(3):471-483.e7
The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly.
Wrobel L, Sokol AM, Chojnacka M, Chacinska A
Scientific reports 2016 Jun 6;6:27484
Scientific reports 2016 Jun 6;6:27484
TIM29 is a subunit of the human carrier translocase required for protein transport.
Callegari S, Richter F, Chojnacka K, Jans DC, Lorenzi I, Pacheu-Grau D, Jakobs S, Lenz C, Urlaub H, Dudek J, Chacinska A, Rehling P
FEBS letters 2016 Dec;590(23):4147-4158
FEBS letters 2016 Dec;590(23):4147-4158
The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.
Wang W, Wang L, Lu J, Siedlak SL, Fujioka H, Liang J, Jiang S, Ma X, Jiang Z, da Rocha EL, Sheng M, Choi H, Lerou PH, Li H, Wang X
Nature medicine 2016 Aug;22(8):869-78
Nature medicine 2016 Aug;22(8):869-78
Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.
Li HY, Zhang LK, Zhu XJ, Shang J, Chen X, Zhu Y, Guo L
Proteomics 2015 Nov;15(21):3629-43
Proteomics 2015 Nov;15(21):3629-43
DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.
Richter-Dennerlein R, Korwitz A, Haag M, Tatsuta T, Dargazanli S, Baker M, Decker T, Lamkemeyer T, Rugarli EI, Langer T
Cell metabolism 2014 Jul 1;20(1):158-71
Cell metabolism 2014 Jul 1;20(1):158-71
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 14927-1-AP(TIMM22 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 14927-1-AP(TIMM22 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The TIMM22 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human TIMM22. This antibody recognizes human, mouse, rat antigen. The TIMM22 antibody has been validated for the following applications: ELISA, WB, IHC analysis.