Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- A700-027-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LAG3 Recombinant Rabbit Monoclonal Antibody (BLR027F)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Antibody clone number
- BLR027F
- Concentration
- 0.50 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LAG3 by WB of immunoprecipitates from HDLM-2 lysate. Antibodies: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (A700-027) and rabbit anti-LAG3 recombinant monoclonal [BLR028F] A700-028. Secondary: ReliaBLOT® reagents (WB120).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LAG3 by WB of HDLM-2, RPMI-8226, Jurkat, MJ [G11], and 293T lysate. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (A700-027). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-GAPDH (A300-641A).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LAG3 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (A700-027).Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] (Product # A700-027 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] (Product # A700-027 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LAG3 in FFPE tonsil by IHC.Antibody: Rabbit anti-LAG3 recombinant monoclonal [BLR027F] (A700-027), Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Lag3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-Lag3 recombinant monoclonal antibody [BLR027F] (Product #A700-027) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).