11835-1-AP

antibody from Invitrogen Antibodies

Targeting: MLF2 NTN4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11835-1-AP

Provider

Invitrogen Antibodies

Product name

MLF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MFRFMRDVE PEDPMFLMDP FAIHRQHMSR MLSGGFGYSP FLSITDGNMP GTRPASRRMQ QAGAVSPFGM LGMSGGFMDM FGMMNDMIGN MEHMTAGGNC QTFSSSTVIS YSNTGDGAPK VYQETSEMRS APGGIRETRR TVRDSDSGLE QMSIGHHIRD RAHILQRSRN HRTGDQEERQ DYINLDESEA AAFDDEWRRE TSRFRQQRPL EFRRLESSGA GGRRAEGPPR LAIQGPEDSP SRQSRRYDW (1-248 aa encoded by BC000898 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 11835-1-AP (MLF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 11835-1-AP (MLF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 11835-1-AP (MLF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11835-1-AP ( MLF2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11835-1-AP ( MLF2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).