Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM09064PU-S - Provider product page
- Provider
- OriGene
- Product name
- Adipose Triglyceride Lipase (PNPLA2) mouse monoclonal antibody, clone AT2G2, Purified
- Antibody type
- Monoclonal
- Description
- Adipose Triglyceride Lipase (PNPLA2) mouse monoclonal antibody, clone AT2G2, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PNPLA2
- Isotype
- IgG
- Antibody clone number
- AT2G2
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: The extracts of mouse kidney (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human ATGL (1/1000) (. . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: Paraffin embedded sections of human breast cancer (left) and prostatic cancer (right) tissue were incubated with anti-human ATGL . (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer. Diaminobenzidine (DAB) was used for detection
- Validation comment
- IHC