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LearnMAB3210
antibody from Abnova Corporation
Targeting: PNPLA2
ATGL, desnutrin, FP17548, iPLA2zeta, TTS-2.2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB3210 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3210, RRID:AB_10555906
- Product name
- PNPLA2 monoclonal antibody, clone AT2G2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant PNPLA2 .
- Isotype
- IgG
- Antibody clone number
- AT2G2
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Human testicular peritubular cells secrete pigment epithelium-derived factor (PEDF), which may be responsible for the avascularity of the seminiferous tubules.
The gene encoding adipose triglyceride lipase (PNPLA2) is mutated in neutral lipid storage disease with myopathy.
ATGL has a key role in lipid droplet/adiposome degradation in mammalian cells.
Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase.
Windschüttl S, Kampfer C, Mayer C, Flenkenthaler F, Fröhlich T, Schwarzer JU, Köhn FM, Urbanski H, Arnold GJ, Mayerhofer A
Scientific reports 2015 Sep 3;5:12820
Scientific reports 2015 Sep 3;5:12820
The gene encoding adipose triglyceride lipase (PNPLA2) is mutated in neutral lipid storage disease with myopathy.
Fischer J, Lefèvre C, Morava E, Mussini JM, Laforêt P, Negre-Salvayre A, Lathrop M, Salvayre R
Nature genetics 2007 Jan;39(1):28-30
Nature genetics 2007 Jan;39(1):28-30
ATGL has a key role in lipid droplet/adiposome degradation in mammalian cells.
Smirnova E, Goldberg EB, Makarova KS, Lin L, Brown WJ, Jackson CL
EMBO reports 2006 Jan;7(1):106-13
EMBO reports 2006 Jan;7(1):106-13
Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase.
Zimmermann R, Strauss JG, Haemmerle G, Schoiswohl G, Birner-Gruenberger R, Riederer M, Lass A, Neuberger G, Eisenhaber F, Hermetter A, Zechner R
Science (New York, N.Y.) 2004 Nov 19;306(5700):1383-6
Science (New York, N.Y.) 2004 Nov 19;306(5700):1383-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The extracts of mouse kidney (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with PNPLA2 monoclonal antibody, clone AT2G2 (Cat # MAB3210 ; 1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Paraffin embedded sections of human breast (A) and prostatic cancer tissue (B) were incubated with PNPLA2 monoclonal antibody, clone AT2G2 (Cat # MAB3210 ; 1 : 25) for 2 hours at room temperature.Antigen retrieval was performed in 100 mM sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
