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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 15376-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPTLC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MATATEQWV LVEMVQALYE APAYHLILEG ILILWIIRLL FSKTYKLQER SDLTVKEKEE LIEEWQPEPL VPPVPKDHPA LNYNIVSGPP SHKTVVNGKE CINFASFNFL GLLDNPRVKA AALASLKKYG VGTCGPRGFY GTFE (1-143 aa encoded by BC007085)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 15376-1-AP (SPTLC1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 15376-1-AP (SPTLC1 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SPTLC1 (IP:15376-1-AP, 4ug; Detection:15376-1-AP 1:500) with HEK-293 cells lysate 880ug.
