Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [6]
- Other assay [1]
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Validation data
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- Product number
- 19112-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GOLPH3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTSLTQRSS GLVQRRTEAS RNAADKERAA GGGAGSSEDD AQSRRDEQDD DDKGDSKETR LTLMEEVLLL GLKDREGYTS FWNDCISSGL RGCMLIELAL RGRLQLEACG MRRKSLLTRK VICKSDAPTG DVLLDEALKH VKETQPPETV QNWIELLSGE TWNPLKLHYQ LRNVRERLAK NLVEKGVLTT EKQNFLLFDM TTHPLTNNNI KQRLIKKVQE AVLDKWVNDP HRMDRRLLAL IYLAHASDVL ENAFAPLLDE QYDLATKRVR QLLDLDPEVE CLKANTNEVL WAVVAAFTK (1-298 aa encoded by BC033725 )
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat testis tissue were subjected to SDS PAGE followed by western blot with 19112-1-AP (GOLPH3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 19112-1-AP (GOLPH3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 19112-1-AP ( GOLPH3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 19112-1-AP (GOLPH3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 19112-1-AP (GOLPH3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 19112-1-AP (GOLPH3 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 19112-1-AP (GOLPH3 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GOLPH3 (IP:19112-1-AP, 3ug; Detection:19112-1-AP 1:1500) with mouse testis tissue lysate 4000ug.