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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005435-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005435-M02, RRID:AB_11188381
- Product name
- Mouse Monoclonal POLR2F Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. This product is specific for Human POLR2F monoclonal antibody (M02), clone 2G2 [Gene ID: 5435].
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POLR2F Antibody (2G2) [H00005435-M02] - Analysis of POLR2F expression in transfected 293T cell line by POLR2F monoclonal antibody (M02), clone 2G2.Lane 1: POLR2F transfected lysate (Predicted MW: 14.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: POLR2F Antibody (2G2) [H00005435-M02] - Detection limit for recombinant GST tagged POLR2F is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: POLR2F Antibody (2G2) [H00005435-M02] - Analysis of monoclonal antibody to POLR2F on HeLa cell . Antibody concentration 10 ug/ml.
