HPA000827

antibody from Atlas Antibodies

Targeting: POLR2F HRBP14.4, RPB6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA000827

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000827, RRID:AB_1079834

Product name

Anti-POLR2F

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

GDDFDDVEEDEGLDDLENAEEEGQENVEILPSGER
PQANQKRITTPYMTKYERARVLGTRALQIAMCAPV
MVELEGETDPLLIAMKELKARKIPIIIRRYLPDGS
YEDWGVDELII


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and POLR2F over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411849).

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & nucleoli fibrillar center.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells, cells in molecular layer and cells in granular layer.

Sample type

HUMAN