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- Antigen structure
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- Validations
- Immunohistochemistry [2]
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- Product number
- AF4237 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal SorCS2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse SorCS2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) SorCS2 and less than 5% cross-reactivity with rhSorCS1 and recombinant mouse SorCS1 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references SorCS2 Controls Functional Expression of Amino Acid Transporter EAAT3 and Protects Neurons from Oxidative Stress and Epilepsy-Induced Pathology.
SorCS2-mediated NR2A trafficking regulates motor deficits in Huntington's disease.
Disruption of SorCS2 reveals differences in the regulation of stereociliary bundle formation between hair cell types in the inner ear.
Prions amplify through degradation of the VPS10P sorting receptor sortilin.
Malik AR, Szydlowska K, Nizinska K, Asaro A, van Vliet EA, Popp O, Dittmar G, Fritsche-Guenther R, Kirwan JA, Nykjaer A, Lukasiuk K, Aronica E, Willnow TE
Cell reports 2019 Mar 5;26(10):2792-2804.e6
Cell reports 2019 Mar 5;26(10):2792-2804.e6
SorCS2-mediated NR2A trafficking regulates motor deficits in Huntington's disease.
Ma Q, Yang J, Milner TA, Vonsattel JG, Palko ME, Tessarollo L, Hempstead BL
JCI insight 2017 May 4;2(9)
JCI insight 2017 May 4;2(9)
Disruption of SorCS2 reveals differences in the regulation of stereociliary bundle formation between hair cell types in the inner ear.
Forge A, Taylor RR, Dawson SJ, Lovett M, Jagger DJ
PLoS genetics 2017 Mar;13(3):e1006692
PLoS genetics 2017 Mar;13(3):e1006692
Prions amplify through degradation of the VPS10P sorting receptor sortilin.
Uchiyama K, Tomita M, Yano M, Chida J, Hara H, Das NR, Nykjaer A, Sakaguchi S
PLoS pathogens 2017 Jun;13(6):e1006470
PLoS pathogens 2017 Jun;13(6):e1006470
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- SorCS2 in Mouse Brain. SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- SorCS2 in Mouse Brain. SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
