PA5-26838

antibody from Invitrogen Antibodies

Targeting: GLG1 CFR-1, ESL-1, MG-160

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-26838

Provider

Invitrogen Antibodies

Product name

GLG1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with chicken, hamster and rat based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a GLG1 polyclonal antibody (Product # PA5-26838) in HepG2, T47D cell lysates (35 µg per lane).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a GLG1 polyclonal antibody (Product # PA5-26838) in HepG2, T47D cell lysates (35 µg per lane).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a GLG1 polyclonal antibody (Product # PA5-26838) in mouse cerebellum tissue lysates (35 µg per lane).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HepG2 (Lane 1), HepG2 treated with Monensin (10uM for 90 minutes) (Lane 2), MCF7 (Lane 3), T-47D (Lane 4), A549 (Lane 5), THP-1 (Lane 6), HeLa (Lane 7), tissue extracts of Mouse Heart (Lane 8) and Mouse Testis (Lane 9). The blot was probed with GLG1 Polyclonal Antibody (Product # PA5-26838, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A band at ~160 kDa corresponding to GLG1 was observed across all the cell lines and tissues tested and reduced upon treatment with Monensin in HepG2.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GLG1 was performed using 70% confluent log phase Hep G2 cells treated with 10µM of Monensin for 90 mins. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GLG1 Rabbit Polyclonal Antibody (Product # PA5-26838) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962).8). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Golgi apparatus localization. Panel e shows Monensin treated cells with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded skeletal muscle using a GLG1 polyclonal antibody (Product # PA5-26838), followed by HRP-conjugated secondary antibody and DAB staining.