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- References [1]
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- Flow cytometry [1]
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- Product number
- MAB253 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-5R alpha/CD125 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-5 R alpha/CD125 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) IL-5 R beta , rhIL-9 R, rhIL-4 R, rmIL-5 R alpha , or rhIL-13 R alpha 1.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Myeloid-derived suppressor cells in the peripheral blood of cancer patients contain a subset of immature neutrophils with impaired migratory properties.
Brandau S, Trellakis S, Bruderek K, Schmaltz D, Steller G, Elian M, Suttmann H, Schenck M, Welling J, Zabel P, Lang S
Journal of leukocyte biology 2011 Feb;89(2):311-7
Journal of leukocyte biology 2011 Feb;89(2):311-7
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of IL-5 R alpha/CD125 in HEK293 human embryonic kidney cell line transfected with human IL-5 R alpha/CD125 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human IL-5 R alpha/CD125 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human IL-5 R alpha/CD125 Monoclonal Antibody (Catalog # MAB253) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
