GTX630779

antibody from GeneTex

Targeting: TPI1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX630779

Provider

GeneTex

Product name

Triosephosphate isomerase antibody [GT181]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:500.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod no

Proprietary.

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod page

KD Reagent Prod page

Antibody Lot Number

41631

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.A. 30 £gg 293T whole cell extract B. 30 £gg A431 whole cell extract C. 30 £gg HeLa whole cell extract D. 30 £gg HepG2 whole cell extract12 % SDS-PAGETriosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.A. 30 £gg Neuro2A whole cell extractB. 30 £gg C8D30 whole cell extractC. 30 £gg NIH-3T3 whole cell extractD. 30 £gg Raw264.7 whole cell extractE. 30 £gg C2C12 whole cell extract12 % SDS-PAGETriosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.A. 30 £gg PC-12 whole cell extractB. 30 £gg Rat2 whole cell extract12 % SDS-PAGETriosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Triosephosphate isomerase protein stained by Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.