NBP1-31470

antibody from Novus Biologicals

Targeting: TPI1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NBP1-31470

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-31470, RRID:AB_2287730

Product name

Rabbit Polyclonal Triosephosphate isomerase Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Quantification of biomarkers for beef meat qualities using a combination of Parallel Reaction Monitoring- and antibody-based proteomics.

Bonnet M, Soulat J, Bons J, Léger S, De Koning L, Carapito C, Picard B
Food chemistry 2020 Jul 1;317:126376

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Beef tenderness and intramuscular fat proteomic biomarkers: Effect of gender and rearing practices.

Picard B, Gagaoua M, Al Jammas M, Bonnet M
Journal of proteomics 2019 May 30;200:1-10

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Beef tenderness and intramuscular fat proteomic biomarkers: muscle type effect.

Picard B, Gagaoua M, Al-Jammas M, De Koning L, Valais A, Bonnet M
PeerJ 2018;6:e4891

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Reverse Phase Protein array for the quantification and validation of protein biomarkers of beef qualities: The case of meat color from Charolais breed.

Gagaoua M, Bonnet M, De Koning L, Picard B
Meat science 2018 Nov;145:308-319

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Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses.

Gagaoua M, Bonnet M, Ellies-Oury MP, De Koning L, Picard B
Food chemistry 2018 Jun 1;250:245-252

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Proteomic analysis of Pteropus alecto kidney cells in response to the viral mimic, Poly I:C.

Mok L, Wynne JW, Ford K, Shiell B, Bacic A, Michalski WP
Proteome science 2015;13:25

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Externalized glycolytic enzymes are novel, conserved, and early biomarkers of apoptosis.

Ucker DS, Jain MR, Pattabiraman G, Palasiewicz K, Birge RB, Li H
The Journal of biological chemistry 2012 Mar 23;287(13):10325-43

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and blotted with Triosephosphate isomerase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat and human cells. Immunodetection of Eno1, Tpi1, Pgam1 and beta2-Tub (load control) in Poly I:C transfected and Control PaKiT03 cells at 4, 8 and 20 hpt. Image collected and cropped by CiteAb from the following publication (http://www.proteomesci.com/content/13/1/25), licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NBP1-31470] - Paraffin-embedded SNU-16 xenograft, using antibody at 1:100 dilution.