- GTX105085 - GeneTex LOXL2 antibody

Submitted references Aberrant Collagen Cross-linking in Human Oral Squamous Cell Carcinoma.

Saito T, Uzawa K, Terajima M, Shiiba M, Amelio AL, Tanzawa H, Yamauchi M
Journal of dental research 2019 May;98(5):517-525

Multiple Functions of Lysyl Oxidase Like-2 in Oral Fibroproliferative Processes.

Saxena D, Mahjour F, Findlay AD, Mously EA, Kantarci A, Trackman PC
Journal of dental research 2018 Oct;97(11):1277-1284

Comparative analysis of lysyl oxidase (like) family members in pulmonary fibrosis.

Aumiller V, Strobel B, Romeike M, Schuler M, Stierstorfer BE, Kreuz S
Scientific reports 2017 Mar 10;7(1):149

Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.

Alshenibr W, Tashkandi MM, Alsaqer SF, Alkheriji Y, Wise A, Fulzele S, Mehra P, Goldring MB, Gerstenfeld LC, Bais MV
Arthritis research & therapy 2017 Aug 2;19(1):179

HIF-1α promoted vasculogenic mimicry formation in hepatocellular carcinoma through LOXL2 up-regulation in hypoxic tumor microenvironment.

Wang M, Zhao X, Zhu D, Liu T, Liang X, Liu F, Zhang Y, Dong X, Sun B
Journal of experimental & clinical cancer research : CR 2017 Apr 27;36(1):60

Orthotopic non-metastatic and metastatic oral cancer mouse models.

Bais MV, Kukuruzinska M, Trackman PC
Oral oncology 2015 May;51(5):476-82

The role of lysyl oxidase-like 2 in the odontogenic differentiation of human dental pulp stem cells.

Kim JH, Lee EH, Park HJ, Park EK, Kwon TG, Shin HI, Cho JY
Molecules and cells 2013 Jun;35(6):543-9

Novel application of human periodontal ligament stem cells and water-soluble chitin for collagen tissue regeneration: in vitro and in vivo investigations.

Jung IH, Park JC, Kim JC, Jeon DW, Choi SH, Cho KS, Im GI, Kim BS, Kim CS
Tissue engineering. Part A 2012 Mar;18(5-6):643-53

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE GTX105085 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects LOXL2 protein by Western blot analysis.A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract7.5 % SDS-PAGELOXL2 antibody (GTX105085) dilution: 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) C: H1299 7.5% SDS PAGE GTX105085 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects LOXL2 protein by western blot analysis.A. 30 ?g A431 whole cell lysate/extract B. 30 ?g HeLa whole cell lysate/extract C. 30 ?g HepG2 whole cell lysate/extract7.5 % SDS-PAGELOXL2 antibody (GTX105085) dilution: 1:500
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody validation by shRNA knock-down.Upperpanel: LOXL2 antibody (GTX105085)Lower panel: beta Actin antibody (GTX110564)A. 30 ?g HeLa whole cell lysate/extract B. 30 ?g whole cell lysate/extract of LOXL2 shRNA-transfected HeLa cellsC. 30 ?g whole cell lysate/extract of LOXL2 shRNA-transfected HeLa cells7.5 % SDS-PAGELOXL2 antibody (GTX105085) dilution: 1:500beta Actin antibody (GTX110564) dilution: 1:20000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects LOXL2 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract7.5 % SDS-PAGELOXL2 antibody (GTX105085) dilution: 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects LOXL2 protein by western blot analysis. MDA-MB-231 whole cell extracts and LOXL2-transfected (+, including 3XFlag-tag) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted by 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects LOXL2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at a dilution of 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects LOXL2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects LOXL2 protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. (PMID 21732535)

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: LOXL2 protein stained by LOXL2 antibody (GTX105085) diluted at 1:500..Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects LOXL2 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. LOXL2 antibody (GTX105085) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects LOXL2 protein at cytoplasm and membrane in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. LOXL2 antibody (GTX105085) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: LOXL2 antibody detects secreted LOXL2 protein in mouse kidney by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.LOXL2 antibody (GTX105085) diluted at 1:500.

GTX105085

Antibody data